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Therapeutic Potential of Canine Bone Marrow Derived Mesenchymal Stem Cells and its Conditioned Media in Diabetic Rat Wound Healing

Mohd Matin Ansari, Sreekumar TR, Vikash Chandra, Pawan K Dubey, G Sai Kumar, Amarpal and G Taru Sharma

Present study was conducted to explore the possibility of treating the diabetic rat wounds by canine bone marrow derived mesenchymal stem cells (BMSCs) and its conditioned media (CM). Canine bone marrow stem cells were isolated ascetically from dog and cultured in vitro. Stem cells conditioned media was collected from third passage cells at 120 hours of culture. Streptozotocin was used for induction of diabetes in rats. Six groups were made for the wound healing therapy in which group I, II and III were non-diabetic while group IV, V and VI were diabetic with six animals in each group and one wound was created in each animal. Group II and V received stem cells in culture media and group III and VI received conditioned media while group I and IV were kept as respective controls given only stem cell culture media. Stem cells and its conditioned media were injected at the periphery of wounds. Wound healing was assessed by wound contraction, photographic evaluation at different time interval (0, 3rd, 7th, 14th, 21st and 28th day) and histomorphological examination on day 28. The outcomes of wound healing experiment suggested that canine bone marrow stem cells as well as its conditioned media can be exploited xenogenically very well for diabetic rat wound healing.