Bioquímica e Bioquímica Analítica

Abstrato

Synthesis of the New 2-[6-Nitro 2-Benzothiazolylazo]-4-Hydroxy Benzoic Acid Organic Reagent for Spectrophotometric Determination of Copper(II)

Aqeel Mahdi Jreo

A new 2-[6-Nitro-2-benzothiazolylazo]-4-hydroxy benzoic acid (NO2BTAHB) organic reagent was synthesized. A sensitive and selective spectrophotometric method was proposed for the rapid determination of Cu(II) using (NO2BTAHB) reagent. The reaction between Cu(II) and (NO2BTAHB) reagent is instantaneous at pH=6.0 and the absorbance remains stable for over 24 hrs. The Method allows for the determination of Cu(II) over the range (0.1-6.0) μg.ml-1, with molar absorptivity of (7.45 × 10+3)l.mol-1.cm-1 and a detection limit of 0.0245 μg.ml-1. Recovery and relative error values of precision and accuracy of method were found to be R.S.D=1.7%, Re=98.6%, and Erel=-1.4%. The properties of complex was studied and show; (M:R) ratio was 1:2 at pH=6.0, and the stability constant of 7.796 × 10+9 L2.mol-2. The interferences of ions (Ni2+, CrO2-, Ca2+, pb2+, Cu+2, WO4 -2, MO4 -2, Co2+, Mg2+, Cd2+, Ba2+, Bi3+) and masking agents effect on absorbance were studied.