Jornal de Nanomedicina e Nanotecnologia

Abstrato

Study DNA Damage after Photodynamic Therapy using Silver Nanoparticles with A549 cell line

Ahmed El-Hussein

Lung Cancer is considered one of the major health problems worldwide. Nanomedicine is considered one of the promising research applications nowadays. This is due to the unique physical and chemical properties of the nanoscale elements. Silver nanoparticles (Ag NPs) in specific has been extensively studied recently in many biomedical applications especially in cancers, since they possess multifunctional effects that make these nanostructures ideal candidates for biomedical applications. Ag NPs were proved to have anti- tumor activity with apoptotic cell death pathway. The goal of the current work was to investigate the degree of DNA damage that results from the usage of Ag NPs as a photosensitizer (PS) in photodynamic therapy (PDT), besides to evaluate the reactive oxygen species (ROS) yield that was associated. The results showed the occurrence of DNA damage in lung cancer cells (A549) through the generation of ROS via the detection of the mitochondrial membrane potential changes.