Jornal de Nanomedicina e Nanotecnologia

Abstrato

Sex Differential Influence of Acute Orally-administered Silver nanoparticles (Ag-NPs) on Some Biochemical Parameters in Kidney of Mice Mus musculus

Heydrnejad MS and Samani RJ

Our data on the gender effect of Ag-NPs on animals are inconclusive with mixed results. This study, therefore aimed to clarify sex differential influence of acute orally-administered Ag-NPs on some biochemical parameters (Blood Urine Nitrogen (BUN), Creatinine (Cr), Sodium and Potassium) in kidney of mice Mus musculus. A group of BALB/c mice of about 9 weeks (weighting 27.2 ± 3.0 g) were randomly divided into three groups (each with two replications): Ag-NPs (2) and the control (1) groups, each with 9 mice. Almost equal numbers of male and female mice were used in this study. Once a day at the same time, a volume of 50 microliters from the nanosilver solution (20 and 50 ppm) was administered orally at a given time. The untreated (control) group received distilled water without Ag-NPs. Samplings (n = 9) were conducted on days 2, 7 and 14. The blood was obtained directly from heart using heparinized tubes. The serum was obtained by centrifugation of the whole blood at 3000 rpm for 15 min. Ag-NPs had potential to adversely affect the kidney functions in gender-dependent manner so that obvious kidney toxicity was found in female mice. This study suggests that female mice suffer more serious kidney toxicity than male mice when treated with Ag-NPs. Our findings build up necessary knowledge on Ag-NPs in vivo distribution and mechanisms responsible for Ag-NPs meditated toxicity to organisms. Further studies are required to elucidate in depth how Ag-NPs interact and affect cellular processes.