Jornal de Patologia Vegetal e Microbiologia

Abstrato

Potential of Grape Epiphytic Antagonists to Biocontrol Aspergillus Transmission and Accumulation of Aflatoxin B1 and Ochratoxin A in Post Harvest Taify Table Grape

Abd El-Raheem R El-Shanshoury, Yasser H El-Halmouch, Samia F Mohamed and Mervat F Fareed

Taify grape is one of the important summer fruits in the Taif area in Saudi Arabia. A part of grape spoiled because by fungi and their toxins, influencing intersection processing, and export, alongside the quality of superior general welfare risk of exposure. The aim of this work was to check the ability of live cells and crude cell-free extracts of the grape epiphytic antagonists Pseudomonas aeruginosa, Bacillus vallismortis and B. amyloliquefaciens to control the transmission of Aspergillus niger, A. parasiticus and A. tubingensis to post-harvest Taify table grape berries (Vitis vinifera L.). Gathering of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was also evaluated. Also, the activity of peroxidase enzyme, total phenol, and lipid peroxidation evaluated. The healthy and injured grape berry inoculated or suspended in the live bacterium cell suspension or bacteria free extract were challenged by the above fungi and stored at 5°C and 20°C for 28 and 50 days, individually. The treatments were effective for lessening the parasites misuse, spoil advance, and decay rate, and AFB1 and OTA aggregation. A pre-dousing of the in-place grape berries was prevalent. The peroxidase enzyme and the total phenol expanded further, slightness the lipid peroxidation, all reinforce the antagonists. The bacteria, their cell-free extracts, the incited peroxidase enzyme, total phenol and the brought down lipid peroxidation, made the medium ominous to the fungal establishment and further developing, and after that developing aggregate AFB1 and OTA ceased. Additional work expected to recognize the bacterial causal variable as well as the active metabolites.