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Optimization of Process Parameters for the Production of Lipase in Solid State Fermentation by Yarrowia Lipolytica from Niger Seed Oil Cake (Guizotia Abyssinica)

Sarat Babu Imandi, Sita Kumari Karanam and Hanumantha Rao Garapati

The production of extra cellular lipase in Solid State Fermentation (SSF) using Yarrowia lipolytica NCIM 3589 with Niger seed oil cake (Guizotia abyssinica) has been made. Different parameters such as incubation time, inoculum level, initial moisture content, carbon level and nitrogen level of the medium were optimized. Screening of various process variables has been accomplished with the help of Plackett–Burman design. The maximum lipase activity of 26.42 units per gram of dry fermented substrate (U /gds) was observed with the substrate of Niger seed oil cake in four days of fermentation. STATISTICA 6.0 was used for implementing Plackett–Burman design.