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Methyl-Β-Cyclodextrin /CTAB Sensitized Fluorescence Method for the Determination of Levofloxacin
Qiuye Ren and Xiashi Zhu
In this work, the method of methyl-β-cyclodextrin (methyl-β-CD) and cetyltrimethyl ammonium bromide (CTAB) cooperatively sensitized fluorescence analysis to determine levofloxacin (LVFX) was developed. The results were shown that, in the system of methyl-β-cyclodextrin-CTAB, the fluorescence intensity of LVFX increased a lot than in H2O medium. Under the conditions of λex/em=330/507 nm and pH 4.5, the linear range and the detection limit for LVFX were found to be 0.04 ~ 4.0 μg/mL and 0.316 ng/mL, respectively. The mechanism of sensitized fluorescence method was discussed by the Benesi-Hildebrand method and the fluorescence quantum yield of fluorescence. The proposed method has been applied for the determination of LVFX in eye drops real samples with satisfactory recovery.