Jaleta Shuka*, Shimelis Tesfaye, Bilisuma Abebe, Takele Abayineh
The current research work was conducted from November 2019 to August 2020 to isolate, identify and molecularly characterizing Pasteurella multocida from cattle diseased with Hemorrhagic Septicemia (HS) in Bambasi and Assosa districts in Benishangul Gumuz Regional State with the objective of the existing vaccine improvement against Bovine pasturellois diseases in Ethiopia. For this purpose, a total of 30 samples were collected from clinical cases following outbreaks of HS in cattle. The samples were transported to National Veterinary Institute of Ethiopia using transport medium and then processed for isolation of P. Multocida. There were 4 samples isolated as P. Multocida. The isolates were identified by cultural and morphological features, biochemical features and pathogenicity tests. With the aim of additional confirmation by means of Pathogenicity study and inocucity estimation, the 4 isolates were inoculated on rabbits and the 4 (100%) isolates were found to be confirmed as Pasteurella up on post-mortem examination. The study of PCR assay revealed the presence of P. Multocida serotype B2, and absence of P. Multocida serotype E2. In conclusions P. Multocida serotype B2 in the designated area is considered as possible pathogens in resulting HS in cattle. However, further study supported by DNA sequencing is essential to finalize these conclusions. At the end based on the conclusions made, the recommendations were forwarded.