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Isolation and Identifi cation of Bacterial Strains and Study of their Resistance to Heavy Metals and Antibiotics

Korrapati Narasimhulu, P. Sreenivasa Rao and A.Venu Vinod

The main objective of the work was to isolate and identify the bacterial strains and their resistance. A total of 9 strains could be isolated from the sample collected from the oxidation pond stage of waste water treatment of NIT Warangal. From all nine strains isolated, the maximum resistance was shown for chromium (490μg/ml for the strain1) and minimum for nickel (80μg/ml for strain6). The maximum resistance was shown for amoxilcillin (330 μg/ml for the strain8) and minimum for kanamycin (15μg/ml for the strain9). The metal resistant strains isolated from the waste water sample can be used for bioremediation process by construction of bioreactors where the strains can be immobilized for treating waste water effl uents from industrial or domestic sources.