Jornal de Bacteriologia e Parasitologia

Abstrato

In Vitro Effect of Pomegranate Extract on Trichomonas Tenax

Gehad T. El-Sherbini

The incidence of T. tenax in patients with acute ulcerative gingivitis has been demonstrated in several published reports about it. Metronidazole was known as the most effective drug for human trichomoniasis, however, drug resistance and toxicity appeared. This study was designed to investigate the in vitro inhibitory activity of p. granatun ethanol extract on the growth and motility of Trichomonas tenax in comparison to metronidazole. Pomegranate ethanol extract group was divided into four groups with concentration of 12.5, 25, 50, and 100 mg/ml, respectively, metronidazole group and blank control. Metronidazole group was given 10 μg/ml of the drug. Each group had 4 wells with 125 μl T. tenax (2×105 / ml). At 12 hr, 24 hr, 48 hr and 72 hr after drug treatment, the anti-T. tenax effect of pomegranate ethanol extract was tested by microscope counting method. The results showed that the better effect on anti-T. tenax 60% pomegranate ethanol extract group with concentrations of 12.5 mg/ml and 25 mg/ml showed higher anti-T. tenax (P<0.01). The ethanol extract of pomegranate granules has a remarkable effect on T. tenax, and among the groups, 60% ethanol extract shows the best anti-T. tenax activity