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Free Mesenchymal Stem Cell-Associated Exosomes Induce Better Neuroregeneration than Mesenchymal Stem Cells and Neural Differentiated Mesenchymal Stem Cells in Canine Model of Spinal Cord Injury

Amal E. Fares

Aim: The aim of this study is to compare spinal cord regeneration following mesenchymal stem cell injection,
neural-differentiated mesenchymal stem cells injection with that following cell free exosome injection.
Method: 20 dogs were randomly divided into Sham group (dorsal laminectomy only) and experimental group
which were subjected to a clipping contusion of the spinal cord. One week after SCI, GFP labeled BMSCs, NSCs
and MSCs-Exo were transplanted intrathecally to investigate the safety and efficacy of each one in the therapy of
SCI. The effects of the transplanted cells in dogs with SCI were determined using functional neurological scoring,
histopathological and immunohistochemical methods.
Results: Our data demonstrate different therapeutic approaches for SCI as BMSCS, NSCs and MSCs-Exo enhanced
remyelination and augmented neural regeneration, resulting in improved neurological functions. Special attention
is paid to MSCs-Exo as they showed the marked improvement in the grey and white matter structure.
Conclusion: MSCs-Exosomes can be successfully used as a promising treatment for spinal cord regeneration.