Jornal de Pesquisa e Desenvolvimento em Aquicultura

Abstrato

Effect of Mushroom Beta Glucan (MBG) on Immune and Haemocyte Response in Pacific White Shrimp (Litopenaeus vannamei)

Chih-Chiu Yang, Shiu-Nan Chen, Chung-Lun Lu, Sherwin Chen, Kam-Chiu Lai and Wen-Liang Liao

The total haemocyte count (THC), differential haemocyte count (DHC), respiratory bursts, and phenoloxidase (PO) activity were determined in the Pacific white shrimp Litopenaeus vannamei which administrated by feeding with diets containing mushroom beta glucan (MBG) at 0.05% and 0.1%. Results showed that shrimp fed a diet containing 0.05% MGB had significantly increased THC and semi-granular cells ratio at 28 days. Intracellular superoxide anion (O2-) production were significantly increased at 14 days which shrimp fed a diet containing 0.05% MBG and PO activity were significantly increased at 14 days which shrimp fed a diet containing 0.1% MGB but equal with control at 28 days. In previously study, semi-granular cells were found as the primary immune activator with their high levels of granules, enzymes and proteins. In this study, the effectively enhance immune responses and compare to high induced semi-granular cells in Pacific white shrimps concluded that MBG is an effective and powerful immunostimulants for shrimp. For application in aquaculture, MBG administration through dietary administration is also a convenient and useful practical technique in the future.