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Detection and Identification of Avian Influenza Virus by cDNA Microarray

Michele N Maughan, Travis W Bliss, Ida Chung, David L Suarez and Calvin L Keeler Jr

Influenza A viruses consisting of all known 16 HA and 9 NA subtypes have been isolated from birds. We have created a diagnostic avian cDNA microarray containing probes corresponding to the highly conserved matrix (M) gene, and selected hemagglutinin (HA), and neuraminidase (NA) subtypes of AIV. cDNA RT-PCR products from the HA, NA, and M genes of various avian influenza isolates and subtypes were used to create an avian influenza virus (AIV) cDNA microarray. The microarray was evaluated against a panel of AIV isolates in order to appraise its application in AIV detection and identification. Utilizing the M gene as a pan-influenza marker, all 10 samples were identified as being strains of type A influenza. The array was able to correctly HA- and NA-subtype subtype 7 out of 10 test samples. This included correctly identifying, subtyping, and determining the geographic origin of all of the H5 subtypes and the two H7 samples of U.S. origin.

Isenção de responsabilidade: Este resumo foi traduzido usando ferramentas de inteligência artificial e ainda não foi revisado ou verificado