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Age may Influence Dystrophin Expression for Bone Marrow Cells Transplantation in mdx Mice

Chen S, Yao X, Zen Y and Zhang W

Objective: To investigate if age influence dystrophin expression for bone marrow cells (BMCs) transplantation in mdx mice.
Methods: BMCs of C57BL/6 mice(new born) ,1.2 × 107 cells ,were injected intravenously into mdx mice of 6 weeks and 12 weeks of age, the mice were preconditioned with 8 Gy γ ray .
Result: 12 weeks after the transplantation, 16% skeletal muscles fibers of 6 weeks and 7% those of 12 weeks age mdx mice have dystrophin protein expression at the sarcolemma of muscle fibers. RT-PCR and Western blot proved the results.
Conclusion: Young mdx mice transplantated with BMCs show better effect than elder one.